Journal: PLoS ONE
Article Title: Comparative miRNA Expression Profiles in Individuals with Latent and Active Tuberculosis
doi: 10.1371/journal.pone.0025832
Figure Lengend Snippet: We confirmed the gene expression levels of miRNAs using real-time qPCR. We analyzed the expression of 7 miRNAs (hsa-miR-223, hsa-miR-365 hsa-miR-424, and hsa-miR-451, hsa-miR-144, hsa-miR-500 and hsa-miR-21*) selected from the microarray data by qPCR. The 2 −ΔΔCT method was used to normalize the relative gene expression data in the qPCR assay. U6 snRNA was set as the reference gene. The miRNA expression value in one subject with LTBI was normalized to 1. Statistical analysis was performed using the unpaired t-test. ** P <0.01, * P <0.05, NS: not significant.
Article Snippet: MiRNA microarray assays were performed using the Agilent Human miRNA microarray platform (version 3, Agilent Technologies) at Shanghai Biochip Co., Ltd. (Shanghai, China).
Techniques: Expressing, Microarray